產(chǎn)品描述
NFAT GFP Reporter Plasmid是自主研發(fā)的用于檢測(cè)NFAT轉(zhuǎn)錄活性水平為目的的報(bào)告基因。NFAT(Nuclear factor of activated T-cells)轉(zhuǎn)錄因子家族對(duì)細(xì)胞因子基因和其它一些對(duì)**反應(yīng)很關(guān)鍵的基因轉(zhuǎn)錄中起關(guān)鍵作用。
NFAT-GFP報(bào)告基因主要用于檢測(cè)細(xì)胞PKC/Ca++信號(hào)通路中NFAT的轉(zhuǎn)錄活性、**研究以及基因過(guò)表達(dá)和RNAi的表型分析等。
pGMNFAT-GFP是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)NFAT結(jié)合位點(diǎn)，可以高效地檢測(cè)NFAT的激活水平。由于載體采用了GFP作為報(bào)告基因，更便于后續(xù)的檢測(cè)。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔儯黾恿速|(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外，由于質(zhì)粒體積減小，使得NFAT-GFP報(bào)告基因更易于轉(zhuǎn)染。NFAT GFP Reporter Plasmid（NFAT-GFP報(bào)告基因質(zhì)粒）
質(zhì)粒圖譜
NFAT GFP Reporter Plasmid（NFAT-GFP報(bào)告基因質(zhì)粒）pGMNFAT -GFP質(zhì)粒測(cè)序引物
5’-TAGCAAAATAGGCTGTCCC-3’
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃保存。
使用說(shuō)明
pGMNFAT-GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。
注意事項(xiàng)
1）本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
2）為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。
參考文獻(xiàn)NFAT GFP Reporter Plasmid（NFAT-GFP報(bào)告基因質(zhì)粒）
[1] Herum KM, et al. Syndecan-4 signaling via NFAT regulates extracellular matrix production and cardiac myofibroblast differentiation in response to mechanical stress. J Mol Cell Cardiol. 54:73-81(2013).
[2] Awla D, et al. NFATc3 regulates trypsinogen activation, neutrophil recruitment, and tissue damage in acute pancreatitis in mice.Gastroenterology. 143(5):1352-60(2012).
[3] Facundo HT, et al. Jones SPO-GlcNAc signaling is essential for NFAT-mediated transcriptional reprogramming during cardiomyocyte hypertrophy.Am J Physiol Heart Circ Physiol. 302(10):H2122-30(2012).
[4] Finsen AV, et al.Syndecan-4 is essential for development of concentric myocardial hypertrophy via stretch-induced activation of the calcineurin-NFAT pathway.PLoS One. 6(12):e28302(2011).
[5] Minematsu H, et al. Nuclear presence of nuclear factor of activated T cells (NFAT) c3 and c4 is required for Toll-like receptor-activated innate inflammatory response of monocytes/macrophages.Cell Signal. 23(11):1785-93(2011). NFAT GFP Reporter Plasmid（NFAT-GFP報(bào)告基因質(zhì)粒）
