產品描述
NF-κB-GFP報告基因是自主研發的用于檢測NFκB轉錄活性水平為目的的報告基因。 NF-κB基因在炎癥反應、**應答和抑制細胞凋亡等方面有著重要的作用。
NF-κB-GFP報告基因主要應用于NF-κB信號通路的研究和基于NF-κB信號通路的**研究以及癌細胞中NF-KB的調控機制的研究。
pGMNF-ΚB-GFP是改造后的哺乳動物真核表達載體，在其多克隆位點插入了多個NF-κB結合位點，可以高效地檢測NF-κB的激活水平。由于載體采用了GFP作為報告基因，更便于后續的檢測。同時，對載體中預測出的其它轉錄因子以外的結合位點進行了適當的突變，增加了質粒的轉錄因子結合特異性。另外，由于質粒體積減小，使得NF-κB-GFP報告基因更易于轉染。NF-κB GFP Reporter Plasmid（NF-κB-GFP報告基因質粒）
質粒圖譜
NF-κB GFP Reporter Plasmid（NF-κB-GFP報告基因質粒）pGMNF-κB -GFP質粒測序引物
5’-TAGCAAAATAGGCTGTCCC-3’
運輸與保存方法
冰袋運輸。-20℃保存。
使用說明
pGMNF-κB -GFP可以采用常規轉染方法轉染哺乳動物細胞。
TNF-α、IL-1β和LPS等是常見的NF-κB激活劑，可作為NF-κB報告基因的陽性對照。
注意事項
1）本質粒未經允許不得用于任何商業用途，也不得移交給訂貨人實驗室以外的任何人或單位。
2）為了您的健康，實驗操作時請穿實驗服和帶一次性手套。NF-κB GFP Reporter Plasmid（NF-κB-GFP報告基因質粒）
參考文獻
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[5] Nagasaki H, et al.Real-time monitoring of inflammation status in 3T3-L1 adipocytes possessing a secretory Gaussia luciferase gene under the control of nuclear factor-kappa B response element.Biochem Biophys Res Commun.420(3):623-7(2012).
[6] Wang DJ, et al.Regulation of the let-7a-3 promoter by NF-κB.PLoS One. 7(2):e31240(2012).
[7] Tran TA, et al.Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B.PLoS One. 6(8):e23660(2011).
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[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells.Chem Biol Interact. 159(2):117-28(2006).NF-κB GFP Reporter Plasmid（NF-κB-GFP報告基因質粒）
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