Polyclonal Antibody Antibody Antibody Antibody to Lactoferrin Lactoferrin Lactoferrin Lactoferrin (LTF)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: LTF
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Species Species Species Species Reactivity: eactivity: eactivity: eactivity: Human
Ig type: Rabbit IgG
Purification: Purification: Purification: Purification: Antibodies are purified by
target protein affinity chromatography.
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F
Form: Liquid
Size: 100μg
Sample: Recombinant human LTF乳鐵傳遞蛋白(LTF)多克隆抗體 [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant human LTF (Glu542~Gln685) expressed in E.coli. Molecular Molecular Molecular Molecular Weight: Weight: Weight: Weight: 17.8kDa
USCN accession accession accession accession No.: P90780Hu01
Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF- ERYYGYTGA FRCLAENAGD VAFVKDVTVL QNTDGNNNEA
WAKDLKLADF ALLCLDGKRK PVTEARSCHL AMAPNHAVVS RMDKVERLKQ VLLHQQAKFG
RNGSDCPDKF CLFQSETKNL LFNDNTECLA RLHGKTTYEK YLGPQ
[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPEFICITY SPEFICITY SPEFICITY SPEFICITY ]
Anti LTF is a rabbit polyclonal antibody raised against human LTF. This antibody
has been selected for its ability to recognize human LTF in immunohistochemical
staining and western blotting, non cross-reactive with other members of the family. 乳鐵傳遞蛋白(LTF)多克隆抗體[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N,
50% glycerol.
[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant human LTF (Glu542~Gln685)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not
used for other purposes.
Loading Loading Loading Loading buffer: buffer: buffer: buffer: 100mM Tris(PH 8.8), 2%SDS, 200mM NaCl, 50% glycerol, BPB
0.01%, Na3N 0.02%.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.乳鐵傳遞蛋白(LTF)多克隆抗體