Polyclonal Antibody Antibody Antibody Antibody to Myosin IG (MYO1G) (MYO1G) (MYO1G) (MYO1G)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
6th Edition (Revised in March, 2013)
[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: MYO1G, Human
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG
Purification: Purification: Purification: Purification: Affinity Chromatography.
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F
Concentration: Concentration: Concentration: Concentration: 200μg/mL
UOM: 100μg Sample: Recombinant MYO1G, Human
[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant MYO1G (Gly769~Pro1016) expressed in E.coli. USCN Accession Accession Accession Accession No.: P93436Hu01
Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below. 肌球蛋白ⅠG(MYO1G)多克隆抗體MGHHHHHHSG SEF- GR DLVWPLPPAV LQPFQDTCHA LFCRWRARQL VKNIPPSDMP
QIKAKVAAMG ALQGLRQDWG CRRAWARDYL SSATDNPTAS SLFAQRLKTL QDKDGFGAVL
FSSHVRKVNR FHKIRNRALL LTDQHLYKLD PDRQYRVMRA VPLEAVTGLS VTSGGDQLVV
LHARGQDDLV VCLHRSRPPL DNRVGELVGV LAAHCQGEGR TLEVRVSDCI PLSHRGVRRL
ISVEPRPEQP EPDFRCARGS FTLLWP
[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against MYO1G. It has been
selected for its ability to recognize MYO1G in immunohistochemical staining and
western blotting.
[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant MYO1G (Gly769~Pro1016)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemil umes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 肌球蛋白ⅠG(MYO1G)多克隆抗體[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.