**球蛋白G4(IgG4)多克隆抗體Polyclonal Antibody to Immunoglobulin G4 (IgG4) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: IgG4, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant IgG4 (Gln222~Lys327) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPA234Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against IgG4. It has been selected for its ability to recognize IgG4 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.**球蛋白G4(IgG4)多克隆抗體 [ QUALITY CONTROL ] Content: The quality control contains recombinant IgG4 (Gln222~Lys327) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant IgG4, Human PAA234Hu01 Polyclonal Antibody to Immunoglobulin G4 (IgG4) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES Used in Western Blot, Sample: Lane1: Human Serum Lane2: Porcine Serum Lane3: Mouse Serum Primary Ab: 3μg/mL Rabbit Anti-Human IgG4 Ab Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SECab05)**球蛋白G4(IgG4)多克隆抗體

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