載脂蛋白H(APOH)多克隆抗體Polyclonal Antibody to Apolipoprotein H (APOH) Organism Species: Bos taurus; Bovine (Cattle) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: APOH, Bovine Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg Sample: Recombinant APOH, Bovine [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant APOH (Arg21~Cys345) expressed in E.coli. USCN Accession No.: RPA310Bo01 Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-RTCPKPDELP FSTVVPLKRT YEPGEQIVFS CQPGYVSRGG IRRFTCPLTG LWPINTLKCM PRVCPFAGIL ENGTVRYTTF EYPNTISFSC HTGFYLKGAS SAKCTEEGKW SPDLPVCAPI TCPPPPIPKF ASLSVYKPLA GNNSFYGSKA VFKCLPHHAM FGNDTVTCTE HGNWTQLPEC REVRCPFPSR PDNGFVNHPA NPVLYYKDTA TFGCHETYSL DGPEEVECSK FGNWSAQPSC KASCKLSIKR ATVIYEGERV AIQNKFKNGM LHGQKVSFFC KHKEKKCSYT EDAQCIDGTI EIPKCFKEHS SLAFWKTDAS DVKPC [ ANTIBODY SPECIFITY ] 載脂蛋白H(APOH)多克隆抗體The antibody is a rabbit polyclonal antibody raised against APOH. It has been selected for its ability to recognize APOH in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant APOH (Arg21~Cys345) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.載脂蛋白H(APOH)多克隆抗體

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載脂蛋白H(APOH)多克隆抗體