基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體
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產(chǎn)品名稱: 基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體
產(chǎn)品型號(hào): 基質(zhì)金屬蛋白酶15多克隆抗體
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體是針對(duì)MMP15的兔多克隆抗體。在**組織化學(xué)染色和**印跡實(shí)驗(yàn)中能識(shí)別MMP15。基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體豐富產(chǎn)品組合，其中包括超過(guò) 42,000 多種高品質(zhì)抗體，并配套提供種類(lèi)齊全的抗體相關(guān)產(chǎn)品。 我們的抗體測(cè)定結(jié)果經(jīng)全球上千次的引用驗(yàn)證，有助于您在一系列抗體應(yīng)用中獲得優(yōu)異的實(shí)驗(yàn)結(jié)果。
基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體
的詳細(xì)介紹
基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體
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來(lái)源多抗制備
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宿主兔
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效價(jià)-
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Ig類(lèi)型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體無(wú)標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體Polyclonal Antibody to Matrix Metalloproteinase 15 (MMP15)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: MMP15, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant MMP15 (Phe280~Asp497) expressed in E.coli. Accession No.: RPA322Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-F YQWKDVDNFK
LP E D D L R GI Q Q LY G TP D G Q P QP TQ P L P T V T P R RP G RP D H R PP RP P QP P P P
GGK PE RP PK P GPP VQP RAT E RP DQY GP NI C DG D F D TVAM L RGEM F VF KG R
WFW RV RH NRV LD NYPMPIGH FW RG LPGDIS AAYE RQ DG RF VF FKG DRYW L
FREANLEPGY PQPLTSYGLG IPYDRIDTAI WWEPTGHTFF FQEDRYWRFN EETQRGD
[ ANTIBODY SPECIFITY ] 基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體The antibody is a rabbit polyclonal antibody raised against MMP15. It has been
selected for its ability to recognize MMP15 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant MMP15 (Phe280~Asp497)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
Used in DAB staining on fromalin fixed
paraffin- embedded liver tissue
Used in Western Blot, Sample:
Recombinant MMP15, Human
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.基質(zhì)金屬蛋白酶15(MMP15)多克隆抗體