Notch同源物3(NOTCH3)多克隆抗體 

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• Notch同源物3(NOTCH3)多克隆抗體標記物無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用WB, ICC, IHC-P, IHC-F, ELISA
• Notch同源物3(NOTCH3)多克隆抗體Polyclonal Antibody to Notch Homolog 3 (NOTCH3)
  Organism Species: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: NOTCH3, Human
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200μg/mL
  UOM: 100μg
  Notch同源物3(NOTCH3)多克隆抗體[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant NOTCH3 (Leu2053~Gln2318) expressed in E.coli. Accession No.: RPL147Hu01
  Sequence: The target protein is fused with two N-terminal Tags, His-tag and
  GST-tag and its sequence is listed below.
  MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
  GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
  DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
  KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
  GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-LKAAQSGS
  KK S R R P P GK A G L GP Q GP R G R GK K LT L A C P G PL A D S S V T L S PV D S L DS P R P
  FGGPPASPGG FPLEGPYAAA TATAVSLAQL GGPGRAGLGR QPPGGCVLSL GLLNPVAVPL
  DWARLPPPAP PGPSFLLPLA PGPQLLNPGT PVSPQERPPP YLAVPGHGEE YPAAGAHSSP
  PKARFLRVPS EHPYLTPSPE SPEHWASPSP PSLSDWSEST PSPATATGAM ATTTGALPAQ
  PLPLSVPSSL AQAQTQLGPQ PEVTPKRQ
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against NOTCH3. It has been
  selected for its ability to recognize NOTCH3 in immunohistochemical staining and
  western blotting.
  [ APPLICATIONS ]
  Western blotting: 1:100-400
  Immunocytochemistry in formalin fixed cells: 1:100-500
  Immunohistochemistry in formalin fixed frozen section: 1:100-500
  Immunohistochemistry in paraffin section: 1:50-200
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  Notch同源物3(NOTCH3)多克隆抗體[ IMAGES ]
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant NOTCH3 (Leu2053~Gln2318)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Used in DAB staining on fromalin fixed
  paraffin- embedded Lung tissue
  Used in Western Blot, Sample:
  Recombinant NOTCH3, Human
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.