FK506結(jié)合蛋白12雷帕霉素關(guān)聯(lián)蛋白(FRAP)多克隆抗體 

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• FK506結(jié)合蛋白12雷帕霉素關(guān)聯(lián)蛋白(FRAP)多克隆抗體標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
• FK506結(jié)合蛋白12雷帕霉素關(guān)聯(lián)蛋白(FRAP)多克隆抗體Polyclonal Antibody to FK506 Binding Protein 12
  Rapamycin Associated Protein (FRAP)
  Organism Species: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: FRAP, Human
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200μg/mL
  UOM: 100μg
  FK506結(jié)合蛋白12雷帕霉素關(guān)聯(lián)蛋白(FRAP)多克隆抗體[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant FRAP (Ala2226~Val2488) expressed in E.coli. Accession No.: RPB806Hu01
  Sequence: The target protein is fused with two N-terminal Tags, His-tag and
  T7-tag and its sequence is listed below.
  MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - AVIPL STNSGLIGWV
  PHCDTLHALI RDYREKKKIL LNIEHRIMLR MAPDYDHLTL MQKVEVFEHA VNNTAGDDLA
  KLLWLKSPSS EVWFDRRTNY TRSLAVMSMV GYILGLGDRH PSNLMLDRLS GKILHIDFGD
  CFEVAMTREK FPEKIPFRLT RMLTNAMEVT GLDGNYRITC HTVMEVLREH KDSVMAVLEA
  FVYDPLLNWR LMDTNTKGNK RSRTRTDSYS AGQSVEILDG VELGEPAHKK TGTTVPESIH
  SFIGDGLV
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against FRAP. It has been
  selected for its ability to recognize FRAP in immunohistochemical staining and
  western blotting.
  [ APPLICATIONS ]
  Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  [ IMAGES ]
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  FK506結(jié)合蛋白12雷帕霉素關(guān)聯(lián)蛋白(FRAP)多克隆抗體[ QUALITY CONTROL ]
  Content: The quality control contains recombinant FRAP (Ala2226~Val2488)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  Used in DAB staining on fromalin fixed
  paraffin-embedded stomach tissue
  Used in Western Blot, Sample:
  Recombinant FRAP, Human