PC-12細(xì)胞細(xì)胞
生長狀態(tài)： 懸浮生長
品系： Sprague-Dawley
數(shù)量： 大量
ATCC Number： CRL-1603?
相關(guān)**： 其他**
運(yùn)輸方式： 凍存運(yùn)輸
器官來源： 肝
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
Designations: N1-S1 Fudr
Depositors: JE Becker
PC-12細(xì)胞細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:
Source: Organ: liver
Strain: Sprague-Dawley
Disease: hepatoma; Novikoff hepatoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. PC-12細(xì)胞細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments: The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil.
This line is derivative of N1-S1 (ATCC CRL-1604).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
10% fetal bovine serum
0.05 mM FUdR
0.1% pluronic F68
Subculturing: PC-12細(xì)胞細(xì)胞Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 viable cells/ml and subculture at 1 X 10 exp6 cells/ml (about 3 days). Cells can be grown in Florence flasks on a rotary shaker at 180 to 190 RPM.
References: 1084: Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488
26107: Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PC-12細(xì)胞細(xì)胞PubMed: 197803
