pHOOK1載體基本信息
載體名稱: | pHOOK1 |
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質(zhì)粒類型: | 哺乳動物表達載體;Capture-Tec系統(tǒng);**磁珠 |
高拷貝/低拷貝: | 高拷貝 |
克隆方法: | 限制性內(nèi)切酶,多克隆位點 |
啟動子: | CMV |
載體大小: | 6061 bp |
5' 測序引物及序列: | CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’ |
3' 測序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
載體標簽: | IgK、HA tag (N-term);2XMyc tag (N-term) |
載體抗性: | 氨芐青霉素 |
篩選標記: | 博來霉素 |
克隆菌株: | DH5α 等 |
宿主細胞(系): | 哺乳動物細胞 |
備注: |
pHOOK1載體可以用于: 1. The effect of dominant, negative mutations of growth regulating proteins or toxic gene products 2. The acute effects of constitutive mutations 3. The effect of anti-sense RNA or ribozymes 4. Temporal events along a developmental or signaling pathway 5. Activation or inactivation of downstream genes using differential display 6. You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for: a) Immunoblot analysis of proteins b) Isolation of DNA or RNA c) Flow Cytometry See pHOOK-3 |
產(chǎn)品目錄號: | V690-20 |
穩(wěn)定性: | 瞬表達 |
組成型/誘導型: | 組成型 |
病毒/非病毒: | 非病毒 |