pRI 101 DNA vectors are binary plant transformation vectors intended for foreign gene expression in plant cells.
These agrobacterium-mediated plant transformation vectors carry the 35S promoter of caμliflower mosaic virus (CaMV)
and the 5’ non-coding region (5’-UTR) of the alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a
translation enhancer in plants. There are two types of pRI 101 DNA vectors, the pRI 101-AN DNA vector, which carries
the 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR), and the pRI 101 ON DNA vector, which carries the 5’-UTR of rice ADH (OsADH 5’-UTR).
The pRI 101-AN DNA vector is for dicotyledonous plants such as tobacco or Arabidopsis while the pRI 101-ON DNA vector is for
monocotyledonous plants such as rice.
Features
Agrobacterium-mediated plant transformation
Easy and stable integration of dicot and monocot target genes into plant chromosomes
Increased target gene expression via the 5'-UTR translation enhancer region
Vector versatility for dicotyledonous and monoctyledonous plant cells
