細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-2118?

相關(guān)**： 肝癌

品系： Leghorn

運輸方式： 凍存運輸

數(shù)量： 大量

器官來源： 肝

是否是腫瘤細(xì)胞： 0

物種來源： 其他

LMH/2A細(xì)胞Designations: LMH/2A

Depositors: R Binder, DL Williams

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Gallus gallus

Morphology: epithelial

Source: Organ: liver

Strain: Leghorn

Disease: hepatocellular carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: yolk protein genes, and permits the analysis of estrogen responsive genes involved in the transport of yolk proteins and other nutrients to the oocyte.

NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.

Vessels prepared in this fashion may be used immediately.

Receptors:LMH/2A細(xì)胞 estrogen receptor, positive

Tumorigenic: Yes

Cytogenetic Analysis: triploid; modal number = 116; six marker chromosomes

Comments: LMH/2A is a stably transfected cell line constructed in 1989 by R. Binder from the LMH hepatocellular carcinoma cell line (see ATCC CRL-2117).

By cotransfection with the plasmid pRSVCEO (containing the chicken estrogen receptor gene driven by the Rous sarcoma promoter) and the pRSVNEO plasmid (which confers G418 resistance).

LMH/2A cells express high levels of chicken estrogen receptor.

Apolipoprotein (apoII) gene expression is increased by estrogen 300-fold compared with levels in the low receptor expression parent LMH line.

This cell line is estrogen responsive for the expression of endogenous

yolk protein genes, and permits the analysis of estrogen responsive genes involved in the transport of yolk proteins and other nutrients to the oocyte.

These genes include apolipoprotein B and apoII as well as vitellogenin.

NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.

To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes, and remove the liquid.

Vessels prepared in this fashion may be used immediately.

Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Atmosphere: air, 95%; LMH/2A細(xì)胞carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Flasks must be coated with 0.1% gelatin.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22374: Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000

23063: Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775

23244: Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. LMH/2A細(xì)胞Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707