數(shù)量： 大量
細(xì)胞形態(tài)： 成纖維樣
ATCC Number： CRL-1459?
相關(guān)**： 正常
是否是腫瘤細(xì)胞： 0
物種來源： 人
生長狀態(tài)： 貼壁生長
運(yùn)輸方式： 凍存運(yùn)輸
年限： 2.5 months
器官來源： 結(jié)腸
Designations: CCD-18Co
Depositors: MI Cour
CCD-18Co細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: colon
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 8
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 8
CCD-18Co細(xì)胞THO1: 6,7
TPOX: 8,11
vWA: 15,17
Age: 2.5 months
Gender: female
Ethnicity: Black
Comments: The line begins to senesce at about PDL = 42.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Growth Conditions: Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: CCD-18Co細(xì)胞A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
32969: Hinterleitner TA, et al. Il-1 stimulates intestinal myofibroblast COX gene expression and augments activation of CI- secretion in T84 cells. Am. J. CCD-18Co細(xì)胞Physiol. 271: C1262-C1268, 1996. PubMed: 8897833
