細胞形態： **樣
**類型： IgG3
ATCC Number： HB-8248?
數量： 大量
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
Designations: HT 29/36 [SKI 294/HT 29/36]
HT 29/36細胞Depositors: Sloan-Kettering Institute for Cancer Research
Isotype: IgG3
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human colon adenocarcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HT 29/36細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Comments: Animals were immunized with a human colon cancer cell line (HT-29, see ATCC HTB-38).
The antibody reacts with human colon adenocarcinoma cells.
It does not react with frozen sections of normal ***** tissues.
This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers.
Propagation: HT 29/36細胞ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids and 2% HT, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 3882: Cordon-Cardo C, et al. Monoclonal antibodies to human gastrointestinal cancers and hybridoma method of production of the monoclonal antibodies. HT 29/36細胞US Patent 4,579,827 dated Apr 1 1986
