運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgG2b

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-198?

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

Designations: F4/80

Depositors: S Gordon

Isotype: IgG2b

F4/80細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse macrophage

Permits/Forms: In addition to the MTA mentioned above, F4/80細(xì)胞other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Comments: Animals were immunized with thioglycollate elicited mouse peritoneal macrophages.

Spleen cells were fused with NS1 myeloma cells.

The antibody reacts specifically with mouse macrophages in many tissue sites (liver, spleen, bone marrow, brain), and precipitates a 160000 dalton glycoproteinF4/80細(xì)胞 (function unknown).

At the request of Dr. Gordon, the line was deposited with the ATCC in 1988 by Dr. Ralph Steinman of the Rockefeller University.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1635: Hume DA, et al.F4/80細(xì)胞 The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J. Exp. Med. 158: 1522-1536, 1983. PubMed: 6355361

1636: Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815, 1981. PubMed: 7308288