細胞形態： 成纖維樣
生長狀態： 貼壁生長
數量： 大量
品系： C3H/An
組織來源： subcutaneous connective tissue; areolar and adipose
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
年限： 100 days
ATCC Number： CRL-8985?
相關**： 正常
MTKP 97-12細胞Designations: MTKP 97-12
Depositors: Oncogen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Strain: C3H/An
Tissue: subcutaneous connective tissue; areolar and adipose
Disease: normal
Permits/Forms:MTKP 97-12細胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: produces protein antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Age: 100 days
Gender: male
Comments: The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.3, 2.6; HindIII--2.7; PvuII--0.6, 1.6; SstI--0.4, 1.7, 2.3; SalI--1.3. [111950]
The MTKP 97-12 cell line was produced by transfecting LMTK- cells with the expression plasmid pMTp97b that contains a cDNA sequence coding for the p97 melanoma associated antigen.
The cells produce approximately 4,000,000 molecules of p97 per cell under the control of the mouse metallothionein promoter.
A metallothionein promoter has been added upstream of the cDNA sequence.MTKP 97-12細胞 Expression can be induced by treating with 80 uM ZnCl and 9 uM CdCl for 24 hrs. [111950]
Digestion with EcoRI and HindIII excises the insert in two fragments. The larger fragment contains the complete coding sequence, that extends approximately from nt 60 to nt 2300. [111950]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Twice per week
Remove old medium, add fresh 0.25% trypsin, EDTA (0.02%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detachMTKP 97-12細胞 (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Preservation: culture medium, 50%; fetal bovine serum, 40%; DMSO, 10%
Related Products: plasmid in bacteria:ATCC 53403
References: 40602: Brown JP, et al. Vaccines against melanoma. US Patent 5,141,742 dated Aug 25 1992
111950: Brian W Poor, personal communication
