年限： *****

細胞形態： 上皮樣

ATCC Number： CCL-42?

生長狀態： 貼壁生長

運輸方式： 凍存運輸

數量： 大量

是否是腫瘤細胞： 0

物種來源： 其他

組織來源： connective tissue and muscle

Designations: FHM

FHM細胞Depositors: RG Malsberger

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Pimephales promelas

Morphology: epithelial

Source: Tissue: connective tissue and muscle

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Isolation: Isolation date: April, 1962

Virus Resistance: poliovirus 1

Age: *****

Comments: The FHMFHM細胞 (Fat Head Minnow) line of epithelial cells was derived from tissue posterior to the anus, exclusive of the caudal fin, of normal ***** minnows of both sexes by M. Gravell and R.G. Malsberger, in April, 1962. [22174]

The cells multiply over a wide range of temperatures (0 to 36C) with optimal growth between 28C and 34C.

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Hanks' BSS, 90%; fetal bovine serum, 10%

Atmosphere: air, 100%

Temperature: 34.0°C; (Max. 34.0 °C, Min. 28.0°C)

Subculturing: Protocol: Remove old medium, add fresh 0.25% trypsin solution, rinse and remove. Add 1 to 2ml of trypsin solution, allow the flask to sit at room temperature until the cells detach. FHM細胞Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: At the time of subcultivation (this will vary depending upon the temperature of incubation)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

References: 22174: Gravell M, Malsberger RG. A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 126: 555-565, 1965. PubMed: 5220177

26198: Leibovitz A. FHM細胞The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hyg. 78: 173-180, 1963. PubMed: 14063721