年限： 10 years

是否是腫瘤細胞： 0

物種來源： 人

器官來源： 外周血

生長狀態： 懸浮生長

細胞形態： **樣

ATCC Number： CRL-9591?

運輸方式： 凍存運輸

相關**： 其他**

數量： 大量

Designations: MV-4-11

Depositors: Wistar Institute

Biosafety Level: 1

MV-4-11細胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Disease: biphenotypic B myelomonocytic leukemia

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Applications: transfection host

Antigen Expression: CD4; Homo sapiens

CD10; Homo sapiens

CD15, human; Homo sapiens

CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13

D16S539: 11,12

D5S818: 11,12

D7S820: 8,9

THO1: 8,9.3

TPOX: 8,11

vWA: 14,15

MV-4-11細胞Cytogenetic Analysis: 48, XY, t(4;11)(q21;q23), +8, +19

Age: 10 years

Gender: male

Comments: The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132]. However, this line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line. Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218].

This line was formerly designated ATCC HTB-189.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: MV-4-11細胞If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/ml transferrin, 0.005 mg/ml insulin, and 5 ng/ml GM-CSF.

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

References: 22617: Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132

23467: Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factorsMV-4-11細胞 (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218