ATCC Number： HB-9459?

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

細(xì)胞形態(tài)： **樣

**類(lèi)型： mouse IgG1 kappa

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: CC 49 [CC-49, CC49]

Depositors: The United States of America

Isotype: mouse IgG1 kappa

CC 49細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Cellular Products: immunoglobulin; monoclonal antibody; against TAG-72 human tumor associated glycoprotein

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Comments: CC 49細(xì)胞Animals were immunized with affinity purified TAG-72 from LS 174T cells (see ATCC CL-188).

Spleen cells were fused with NS-1 mouse myeloma cells.

The antibody binds to TAG-72 with an affinity constant of 20 X 10 exp9 liter/mol.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

CC 49細(xì)胞Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

References: 22091: CC 49細(xì)胞Schlom J, Colcher D. Second generation monoclonal antibodies having binding specificity to TAG-72 and human carcinomas and methods for employing the same. US Patent 5,512,443 dated Apr 30 1996