生長狀態(tài)： 貼壁生長
ATCC Number： CRL-2221?
組織來源： epithelium
細(xì)胞類型： 其他細(xì)胞類型
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 人
細(xì)胞形態(tài)： 上皮樣
年限： 70 years *****
器官來源： 前列腺
運(yùn)輸方式： 凍存運(yùn)輸
Designations: PZ-HPV-7
PZ-HPV-7細(xì)胞Depositors: DM Peehl
Biosafety Level: 2 [Cells contain human papilloma viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: prostate
Tissue: epithelium
Cell Type: human papillomavirus 18 (HPV-18) transformed
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.
The cells are negative for prostate specific antigen (PSA).
Tumorigenic: No
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
PZ-HPV-7細(xì)胞D13S317: 12,14
D16S539: 11,12
D5S818: 9,13
D7S820: 9
THO1: 7,9
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: at low passages maintained the diploid karyotype of the normal parental cells but by passage 99 the karyotype had changed to near-triploid.
Age: 70 years *****
Gender: male
Ethnicity: Caucasian
Comments: PZ-HPV-7 was derived from epithelial cells cultured from normal tissue from the peripheral zone of the prostate.
The cells were transformed by transfection with HPV18 DNA.
Incorporation of HPV18 DNA was confirmed by polymerase chain reaction.
Specific amplification of a 160-base pair fragment of the HPV18 E6 transforming region was noted.
PZ-HPV-7細(xì)胞Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.
The cells are negative for prostate specific antigen (PSA).
Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
0.05 mg/ml BPE - provided with the K-SFM kit
5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Temperature: 37.0°C
Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution; let the culture set incubate at 37C for two minutes. Neutralize the trypsin with 0.1% soybean trypsin inhibitor, and gently dislodge the cells by agitating or tapping the flask. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: purified DNA:ATCC CRL-2221D
References: 23116: Weijerman PC, et al. PZ-HPV-7細(xì)胞Lipofection-mediated immortalization of human prostatic epithelial cells of normal and malignant origin using human papillomavirus type 18 DNA. Cancer Res. 54: 5579-5583, 1994. PubMed: 7923200
