是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

器官來源： 肺

ATCC Number： CCL-93?

細(xì)胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: V79-4

Depositors: EH Chu

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

V79-4細(xì)胞Organism: Cricetulus griseus

Morphology: fibroblast

Source: Organ: lung

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Cytogenetic Analysis: modal number = 22; range = 20 to 23, Pseudodiploid. The rate of higher ploidies was 4%. Twelve to fourteen marker chromosomes were common to most cells. These include 1p-, 4q+, 4p+, t(6,?), 7q+ and seven to eight other small markers., Normal N2 and N3 were paired; N1, N5, N6 and N10 were single. Normal X and Y were absent, but a single Xq- was present in every cell.

Gender: male

Comments: This cell line was developed by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster, and was originally designated Strain V.

V79-4細(xì)胞The line was renamed V79 by Elkind in 1958,and was used in studies of X-ray induced damage and repair processes.

In 1966, E.H.Y. Chu obtained the line from W. Sinclair, and isolated the V79-4 clone.

The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours.

A culture at passage 7 after cloning was submitted to the ATCC in July 1988.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:14 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium V79-4細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

derivative:ATCC CRL-1657

References: 21877: . Molecular cell genetics. New York: Wiley; 1985.

22642: Chu EH, et al. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62: 359-377, 1969. PubMed: 5392645

23252: Chu EH, Malling HV. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Natl. Acad. Sci. USA 61: 1306-1312, 1968. PubMed: 5249812

26264: . . Nature 184: 1293-1295, 1959.

26265: Ford DK, Yerganian G. Observations on the chromosomes of Chinese hamster cells in tissue culture. J. Natl. Cancer Inst. 21: 393-425, 1958. PubMed: 13576097

26275: . Molecular cell genetics. New York: Wiley; 1985.

32373: Goodrum FD, et al. V79-4細(xì)胞Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260