ATCC Number： CRL-2404?
運輸方式： 凍存運輸
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 人
生長狀態(tài)： 貼壁生長
年限： 65 year old
器官來源： 皮膚
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
Designations: HEK001
HEK001細(xì)胞Depositors: PB Sugerman, M Bigby
Biosafety Level: 2 [Cells contain human papilloma viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: epithelial
Source: Organ: skin
Cell Type: keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transformed
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1996
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11
D13S317: 8
D16S539: 12,13
HEK001細(xì)胞D5S818: 10,13
D7S820: 12,13
THO1: 6,9
TPOX: 8,9
vWA: 14
Age: 65 year old
Gender: male
Comments: HEK001 cells express keratin 14 but not keratin 10 suggesting that they are proliferating basal-type keratinocytes.
Propagation: ATCC complete growth medium: Keratinocyte-Serum Free medium (GIBCO-BRL 17005-042) with 5 ng/ml human recombinant EGF and 2mM L-glutamine (without bovine pituitary extract and without serum)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
HEK001細(xì)胞Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).
Add 2.0 to 3.0 ml of 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium with 10% FBS added and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 10% FBS and 5% DMSO
Storage temperature: HEK001細(xì)胞liquid nitrogen vapor phase
Related Products: Cell culture tested DMSO:ATCC 4-X
References: 48305: Sugerman PB, Bigby M. Preliminary functional analysis of human epidermal T cells. Arch. Dermatol. Res. 292: 9-15, 2000. PubMed: 10664009
