bEnd.3細(xì)胞

組織來源： cerebral cortex

相關(guān)**： 其他**

ATCC Number： CRL-2299?

品系： BALB/c

生長狀態(tài)： 貼壁生長

物種來源： 小鼠

是否是腫瘤細(xì)胞： 0

細(xì)胞形態(tài)： 內(nèi)皮樣

細(xì)胞類型： 其他細(xì)胞類型

運(yùn)輸方式： 凍存運(yùn)輸

年限： 6 weeks

器官來源： 大腦

數(shù)量： 大量

Designations: bEnd.3

Depositors: EC Butcher

bEnd.3細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: endothelial

Source: Organ: brain

Strain: BALB/c

Tissue: cerebral cortex

Disease: endothelioma

Cell Type: endothelialpolyoma middle T antigen transformed

Cellular Products: von Willebrand factor

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: bEnd.3細(xì)胞The bEnd.3 cell line is provided for research use only under the following terms:1. bEnd.3 cells, or products derived from them, must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, bEnd.3 cells or their products. Commercial interests are the exclusive property of The Max Planck Society. 2. Any proposed commercial use of bEnd.3 cells, or products derived from them, must first be negotiated with Dr. Britta Engelhardt, on behalf of the late Dr. Werner Risau, Max-Planck-Institut fur physiologische und klinische Forschung, W.G. Kerckoff Institut, Abteilung molekulare Zellbiologie, Parkstrasse 1, 61231 Bad Nauheim, Germany. Telephone: 49-6032-705203/265; FAX: 49-6032-072259; email: [email protected]. In all papers reporting any use of bEnd.3 cells, or products derived from them, a direct reference will be made to the original publication (Montesano, et al. Cell 62:435-445, 1990).

Antigen Expression: ICAM-1 +; VCAM-1 +; MAdCAM-1 +

Age: 6 weeks

Comments: The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen. [39039]

The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). [39039]

The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.3 cells by cytokines and lipopolysaccharide (LPS). [39040]

This induction by Tumor Necrosis Factor alpha (TNF alpha), interleukin 1 (IL-1) or LPS is concentration and time dependent. [39040]

MAdCAM-1 is expressed on the surface of unstimulated bEnd.3 cells at early passages but not at passages greater than 30.

Intracellular adhesion molecule 1 (ICAM-1) is constitutively expressed on the cells, and expression is increased by treatment with LPS, IL-1 and TNF alpha.bEnd.3細(xì)胞 [39040]

Vascular cell adhesion molecule 1 (VCAM-1) is constitutively expressed on the cells at early passages but not at passages over 30.

P-selectin can be induced on bEnd.3 cells by Tumor Necrosis Factor alpha (TNF alpha) at both early and late passages but expression is greater at passages over 30.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer just begins to detach. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 39039: Montesano R, et al. Increased proteolytic activity is responsible for the aberrant morphogenetic behavior of endothelial cells expressing the middle T oncogene. bEnd.3細(xì)胞Cell 62: 435-445, 1990. PubMed: 2379237

39040: Sikorski EE, et al. The Peyer's patch high endothelial receptor for lymphocytes, the mucosal vascular addressin, is induced on a murine endothelial cell line by tumor necrosis factor-alpha and IL-1. J. Immunol. 151: 5239-5250, 1993. PubMed: 7693807

39041: Williams RL, et al. Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene. Cell 52: 121-131, 1988. PubMed: 3345558