KG-1細胞
器官來源： 骨髓
生長狀態： 懸浮生長
年限： 59 years
ATCC Number： CRL-8031?
相關**： 其他**
運輸方式： 凍存運輸
細胞形態： **樣
數量： 大量
是否是腫瘤細胞： 0
物種來源： 人
Designations: KG-1
KG-1細胞Depositors: Genentech, Inc.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: bone marrow
Disease: acute myelogenous leukemia
Cellular Products: human leukocyte interferon
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DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Age: 59 years
Gender: male
Ethnicity: Caucasian
KG-1細胞Comments: Responsive to colony stimulating activity (CSA).
Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
See also ATCC CCL-246.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. KG-1細胞Start cultures at 3 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682
4001: Goeddel DV. KG-1細胞Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987
