SUP-B15細胞
生長狀態： 懸浮生長
年限： 8 years
細胞形態： **樣
數量： 大量
細胞類型： B**細胞
是否是腫瘤細胞： 0
物種來源： 人
ATCC Number： CRL-1929?
相關**： 白血病
運輸方式： 凍存運輸
器官來源： 骨髓
Designations: SUP-B15
Depositors: SD Smith
Biosafety Level: 1
SUP-B15細胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: bone marrow
Disease: acute lymphoblastic leukemia
Cell Type: B lymphoblast;
Cellular Products: immunoglobulin (cytoplasmic)
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Antigen Expression: CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR +
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,14
D16S539: 11,12
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,9
vWA: 15,17
SUP-B15細胞Cytogenetic Analysis: 46, XY; the following markers are present: t(9;22)(q34;q11), t(4;14) (p11;q24), der(4)t(1;4) (p11;q33), t(9;22) (q34;q11), der(10)t(3;10) (q25;q26), add(16); Philadelphia chromosome is present.
Age: 8 years
Gender: male
Ethnicity: Caucasian
Comments: This line was was derived from malignant cells collected from the bone marrow of an 8 year old child with Philadelphia chromosome positive B cell ALL.
The cells express multiple B lineage markers, but do not express T cell markers.
The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. [23068]
They are are negative for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), surface immunoglobulin (sIg -) and Epstein-Barr virus. [23068]
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: SUP-B15細胞Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 10 exp5 viable cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: 18 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 22924: Fainstein E, et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330: 386-389, 1987. PubMed: 2825022
22958: Clark SS, et al. Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL). Science 239: 775-777, 1988. PubMed: 3422516
23068: Naumovski L, et al. Philadelphia chromosome-positive acute lymphoblastic leukemia cell lines without classical breakpoint cluster region rearrangement. Cancer Res. 48: 2876-2879, 1988. PubMed: 3162827
23302: Rubin CM, et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 85: 2795-2799, 1988. SUP-B15細胞PubMed: 2833755
23305: Kawasaki ES, et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA 85: 5698-5702, 1988. PubMed: 3165197
