RF/6A細胞
年限： fetus
數量： 大量
ATCC Number： CRL-1780?
相關**： 正常
細胞形態： 上皮樣
運輸方式： 凍存運輸
生長狀態： 貼壁生長
細胞類型： 內皮細胞
是否是腫瘤細胞： 0
物種來源： 獼猴
組織來源： choroid; retina
Designations: RF/6A
RF/6A細胞Depositors: F Hu
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Macaca mulatta
Morphology: epithelial
Source: Tissue: choroid; retina
Disease: normal
Cell Type: endothelial
Cellular Products: factor VIII; fibronectin
Permits/Forms: In addition to the MTA mentioned above, RF/6A細胞other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Age: fetus
Comments: This line spontaneously transformed at an early age and has been passaged over 540 times.
Weibel-Palade granules are seen by electron microscopy.
Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: RF/6A細胞Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22315: Lou DA, Hu FN. RF/6A細胞Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454
