DT40細胞

組織來源： bursa

數量： 大量

運輸方式： 凍存運輸

生長狀態： 懸浮生長

年限： 1 day

是否是腫瘤細胞： 0

物種來源： 其他

細胞形態： **樣

ATCC Number： CRL-2111?

相關**： **瘤

Designations: DT40

Depositors: EH Humphries

Biosafety Level: 1

DT40細胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Gallus gallus

Morphology: lymphoblast

Source: Tissue: bursa

Disease: lymphoma

Cellular Products: immunoglobulin; IgM (surface)

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Applications: transfection host (Roche Transfection Reagents)

Tumorigenic: Yes

Oncogene: c-myc +

Age: 1 day

Comments: DT40細胞DT40 is an avian leukosis virus (ALV) induced bursal lymphoma cell line derived from a Hyline SC chicken.

The original lymphoma was induced by viral infection of a 1 day old chicken with Rous associated virus 1 (RAV-1).

Cell suspensions prepared from tumors that developed within the bursa of Fabricus were transferred intravenously into young syngeneic recipient chickens.

After one transfer in vivo, the DT40 cell line was established.

The cell line contains proviral DNA sequences integrated upstream from the c-myc proto-oncogene and expresses increased levels of c-myc RNA.

It lacks a normal c-myc gene, but contains two copies of an ALV deregulated myc gene.

The cells retain the ability to rearrange the immunoglobulin light chain gene (IgL).

At the IgL locus, DT40 contains one rearranged and one germline allele.

Both the c-rel gene and the v-rel oncogenes induce major histocompatibility (MHC) class II antigen expression on the DT40 cell line.

The expression of MHC class II is induced more rapidly by v-rel than c-rel and several weeks after infection, rel induced class II antigen as much as 50 fold more efficiently than c-rel.

The cell line exhibits a lymphoblastoid phenotype.

Infectivity assays revealed that DT40 releases low levels of infectious RAV-1.

The cells may be used for stable transfection studies.

Propagation: DT40細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 0.05 mM 2-mercaptoethanol, 75%; tryptose phosphate broth, 10%; fetal bovine serum, 10%; chicken serum, 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 2 X 10(5) and 2 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 22571: Hrdlickova R, et al. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J. Virol. 68: 308-319, 1994. PubMed: 8254742

22724: Humphries EH, Zhang G. V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas. Curr. Top. Microbiol. Immunol. 182: 475-483, 1992. PubMed: 1490388

22994: Baba TW, et al. Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151, 1985. PubMed: 2998040

26168: Buerstedde JM, et al. Light chain gene conversion continues at high rate in an ALV-induced cell line. EMBO J. 9: 921-927, 1990. PubMed: 2155784

26170: Kim S, et al. DT40細胞Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line. Mol. Cell. Biol. 10: 3224-3231, 1990. PubMed: 2111450

26171: Buerstedde JM, Takeda S. Increased ratio of targeted to random integration after transfection of chicken B cell lines. Cell 67: 179-188, 1991. PubMed: 1913816