CTX TNA2細胞
生長狀態： 貼壁生長
相關**： 正常
ATCC Number： CRL-2006?
細胞形態： 成纖維樣
物種來源： 大鼠
是否是腫瘤細胞： 0
細胞類型： 其他細胞類型
器官來源： 大腦
數量： 大量
年限： neonate
組織來源： cortex
CTX TNA2細胞品系： Sprague-Dawley
運輸方式： 凍存運輸
Designations: CTX TNA2
Depositors: CF Deschepper
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast
Source: Organ: brain
Strain: Sprague-Dawley
Tissue: cortex
Disease: normal
Cell Type: astrocyte, type 1 phenotype;
Cellular Products: CTX TNA2細胞alpha 2 macroglobulin; transferrin
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Tumorigenic: No
Age: neonate
Comments: The CTX TNA2 cell line was established from primary cultures of type 1 astrocytes from brain frontal cortex tissue of 1 day old rats.
The cultures were transfected 3 days after initial plating with a DNA construct containing the oncogenic early region of SV40 under the transcriptional control of the human GFAP promoter (pGFA-SV-Tt).
And pPGK-neo which contains the murine phosphoglycerate kinase gene promoter.
The transfectants were selected with G418 and cloned.
The cells retain characteristics consistent with the phenotype of type 1 astrocytes.
About 20% of the cells have glial fibrillary acidic protein (GFAP) immunoreactivity.
CTX TNA2細胞The cells have a high affinity uptake mechanism for gamma aminobutyric acid (GABA) that is inhibitable by beta alanine.
The cells produce alpha 2 macroglobulin in amounts similar to those found in primary astrocytes but produce transferrin in much lesser amounts.
This line does not produce proenkephalin A, does not express the O4 or A2B5 epitopes characteristic of type 2 astrocytes, and does not express galactocerebroside.
SV40 T-antigen was found in the nuclei of over 95% of the cells examined by immunostaining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, add fresh 0.25% trypsin, 0.53mM EDTA solution, rinse and remove.
Add fresh trypsin solution, (1 to 2 ml) and allow the cells to sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
NOTE: CTX TNA2細胞The cells will come off in sheets and are difficult to dissociate.
Related Products: Trypsin EDTA Solution: ATCC 30-2101
References: 23333: Radany EH, et al. Directed establishment of rat brain cell lines with the phenotypic characteristics of type 1 astrocytes. Proc. Natl. Acad. Sci. USA 89: 6467-6471, 1992. PubMed: 1378628
