23 ScCr [10ScNCr/23]細胞
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
器官來源： 骨髓
品系： C57BL/10ScN
數量： 大量
ATCC Number： CRL-2751?
年限： 6 months *****
細胞形態： 單核細胞/巨噬細胞
生長狀態： 混合型生長
運輸方式： 凍存運輸
Designations: 23 ScCr [10ScNCr/23]
23 ScCr [10ScNCr/23]細胞Depositors: E Lorenz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Mus musculus
Morphology: macrophage
Source: Organ: bone marrow
Strain: C57BL/10ScN
Cell Type: macrophage
Cellular Products: Macrophage inflammatory protein-2
tumor necrosis factor alpha (TNF alpha)
Permits/Forms: In addition to the MTA mentioned above, 23 ScCr [10ScNCr/23]細胞other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD11a, mouse; Mus musculus
CD11b, mouse; Mus musculus
F4/80, mouse
Age: 6 months *****
Comments: 23 ScCr is a bone marrow derived macrophage cell line developed from a mouse strain with a deletion of the TLR4 locus. The cell line is deficient in toll-like receptor 4 (TLR4). Stimulation with lipoteichoic acid (LTA) induces a five to six fold increase in tumor necrosis factor alpha (TNF alpha) and macrophage inflammatory protein-2 (MIP-2) secretion. No increases in either cytokine were found when cells were stimulated with lipopolysaccharides (LPS).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; 23 ScCr [10ScNCr/23]細胞LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove 75% of the media. Scrape off the attached cells. Resuspend cells at 2 X 10(5) viable cells/ml.Transfer into new flasks.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
23 ScCr [10ScNCr/23]細胞Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 61303: Lorenz E, et al. Toll-like receptor 4 (TLR4)-deficient murine macrophage cell line as an in vitro assay system to show TLR4-independent signaling of Bacteroides fragilis lipopolysaccharide. Infect. Immun. 70: 4892-4896, 2002. PubMed: 12183533
