WI-38細(xì)胞

細(xì)胞類型： 成纖維細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 人

年限： 3 months gestation fetus

ATCC Number： CCL-75?

相關(guān)**： 正常

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

器官來源： 肺

細(xì)胞形態(tài)： 成纖維樣

Designations: WI-38

Depositors: L Hayflick

WI-38細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: testing

transfection host

viruscide testing

Virus Susceptibility: Vesicular stomatitis, Glasgow (Indiana)

Herpes simplex virus

Pseudorabies virus

Human poliovirus 1

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 11

D16S539: 11,12

D5S818: 10

D7S820: 9,11

THO1: 8,9.3

TPOX: 8

vWA: 19,20

WI-38細(xì)胞Cytogenetic Analysis: normal diploid

Isoenzymes: G6PD, B

Age: 3 months gestation fetus

Gender: female

Ethnicity: Caucasian

Comments: The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue.

WI-38 cells have a finite lifetime of 50 plus or minus 10 population doublings with a doubling time of 24 hours.

This line was the first human diploid cell line to be used in human vaccine preparation.

The 8th passage ampule from which this freeze was derived was found to contain a bacterial contaminant (a micrococcus). The cell line was subsequently cured by several passages in the presence of antibiotics.

Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: WI-38細(xì)胞Trypsin and medium should be prewarmed to 37C and the pH should be adjusted to 7.5 before use.

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: growth medium, 95%; DMSO, 5%

Doubling Time: 24 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

22194: Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659

22197: Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085

26220: Hayflick L, et al. Preparation of poliovirus vaccines in a human fetal diploid cell strain. Am. J. Hyg. 75: 240-258, 1962. PubMed: 13905660

26223: . First International Conference on Vaccines against Viral and Rickettsial Diseases of Man. Pan Am. Health Organ. Off. Doc. 147: 581, 1967.

29375: Tomasek JJ, et al. WI-38細(xì)胞Gelatinase A activation Is regulated by the organization of the polymerized actin cytoskeleton. J. Biol. Chem. 272: 7482-7487, 1997. PubMed: 9054450

32244: Hoppe HC, et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed: 9284169

32288: Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

32582: Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591

32790: Debant A, et al. The multidomain protein trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains. Proc. Natl. Acad. Sci. USA 93: 5466-5471, 1996. PubMed: 8643598

32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224

92346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.

92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.