2H-11細胞
品系： C3H/HeJ
年限： *****
組織來源： vascular epithelium
ATCC Number： CRL-2163?
運輸方式： 凍存運輸
數(shù)量： 大量
細胞類型： SV40轉(zhuǎn)化細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
器官來源： 腋窩**結(jié)
Designations: 2H-11
Depositors: KA O'Connell
2H-11細胞Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: axillary lymph node
Strain: C3H/HeJ
Tissue: vascular epithelium
Cell Type: endothelialSV40 transformed
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Tumorigenic: Yes
Antigen Expression: H-2 K; VCAM
Age: *****
Gender: male
Comments: The 2H-11 cell line was derived from a solid tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
The line was cloned in 1992 by limiting dilution.
The cells are sensitive to lysis by activated macrophages as measured in the chromium release assay.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
2H-11細胞The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).
The cells stain positively for SV40 T antigen.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), 2H-11細胞and let the culture sit at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170
23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612
23187: O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. 2H-11細胞PubMed: 1928299
