M3WT5細胞

數量： 大量

是否是腫瘤細胞： 0

物種來源： 倉鼠

器官來源： 卵巢

運輸方式： 凍存運輸

細胞形態： 上皮樣

ATCC Number： CRL-1982?

生長狀態： 貼壁生長

Designations: M3WT5

Depositors: CM Fraser

Biosafety Level: 1

M3WT5細胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus

Morphology: epithelial

Source: Organ: ovary

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Receptors: acetylcholine, muscarinic m3

Gender: female

Comments: M3WT5細胞The M3WT5 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor.

The resulting cells were co-transfected with pMSVNeo and selected for growth in medium containing 0.5 mg/ml G418.

M3WT5 cells are reported to express 690 fmol of receptor protein per mg of membrane protein.

Propagation: ATCC complete growth medium: Ham's F12 medium with 0.05 to 0.1 mg/ml G418, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature (or incubate at 37C) until the cells detach (usually 5 to 10 minutes).

Subcultivation Ratio: M3WT5細胞A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: At the time of subcultivation

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 22692: Buck MA, Fraser CM. Muscarinic acetylcholine receptor subtypes which selectively couple to phospholipase C: pharmacological and biochemical properties. Biochem. Biophys. Res. Commun. M3WT5細胞173: 666-672, 1990. PubMed: 2175603