CMT-93細胞

生長狀態： 貼壁生長

品系： C57BL/icrf

ATCC Number： CCL-223?

相關**： 其他**

數量： 大量

器官來源： 直腸

細胞形態： 上皮樣

是否是腫瘤細胞： 0

物種來源： 小鼠

年限： 19 months

運輸方式： 凍存運輸

Designations: CMT-93

CMT-93細胞Depositors: LM Franks

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Strain: C57BL/icrf

Organ: rectum

Disease: polyploid carcinoma

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Tumorigenic: Yes

Antigen Expression: H-2b

GenoType: a(t)

CMT-93細胞Age: 19 months

Gender: male

Comments: Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. CMT-93細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22753: Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum.CMT-93細胞 J. Pathol. 124: 35-38, 1978. PubMed: 722371