SV40LT-SMC Clone HEP-SA細胞
生長狀態： 貼壁生長
器官來源： 大動脈
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 大鼠
年限： 3 months
運輸方式： 凍存運輸
數量： 大量
品系： Sprague-Dawley
SV40LT-SMC Clone HEP-SA細胞ATCC Number： CRL-2018?
組織來源： smooth muscle
細胞形態： 成纖維樣
Designations: SV40LT-SMC Clone HEP-SA
Depositors: CF Reilly, R McFall
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast
Source: Organ: aorta
Strain: Sprague-Dawley
Tissue: smooth muscle
Cell Type: SV40 transfected
SV40LT-SMC Clone HEP-SA細胞Cellular Products: alpha actin; large T antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 3 months
Comments: This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.
The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin.
Large T antigen is detectable in the nuclei.
Propagation: ATCC complete growth medium: SV40LT-SMC Clone HEP-SA細胞Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90% supplemented with 0.2 mg/ml G418, 90%; bovine calf serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove. Allow the cells to sit at 37C (or at room temperature) until the cells detach (usually 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2030
References: 23008: SV40LT-SMC Clone HEP-SA細胞Reilly CF. Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin. J. Cell. Physiol. 142: 342-351, 1990. PubMed: 2154504
