WRL 68細(xì)胞
ATCC Number： CL-48?
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
器官來(lái)源： 其他
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
數(shù)量： 大量
Designations: WRL 68
Depositors: Burroughs Wellcome Company
WRL 68細(xì)胞Biosafety Level: 2 [Cells contain Human Papilloma Viral (HPV) DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: HeLa contaminant
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
WRL 68細(xì)胞TPOX: 8,12
vWA: 16,18
Isoenzymes: G6PD, A
Comments: A culture submitted to the ATCC in July of 1969 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, WRL 68細(xì)胞and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 3490: Apostolov K. Cell lines. US Patent 3,935,066 dated Jan 27 1976
53333: Gutierrez-Ruiz MC, et al. WRL 68細(xì)胞Expression of some hepatocyte-like functional properties of WRL-68 cells in culture. In Vitro Cell. Dev. Biol. 30A: 366-371, 1994. PubMed: 7522099
