BC3H1細胞
品系： C3H
組織來源： smooth muscle like
生長狀態： 貼壁生長
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
器官來源： 大腦
運輸方式： 凍存運輸
數量： 大量
BC3H1細胞ATCC Number： CRL-1443?
相關**： 腫瘤
Designations: BC3H1
Depositors: W Carlisle
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Organ: brain
Strain: C3H
Tissue: smooth muscle like
BC3H1細胞Disease: tumor
Cell Type: methylnitrosourea induced
Cellular Products: adenylate phosphokinase; creatine phosphokinase
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: acetylcholine
Antigen Expression: H-2k
Comments: Recent data suggest that BC3H1 cells may more closely resemble cells in an arrested state of skeletal muscle differentiation than smooth muscle cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: BC3H1細胞A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22488: Schubert D, et al. Characterization of a unique muscle cell line. J. Cell Biol. 61: 398-413, 1974. PubMed: 4363958
23423: Taubman MB, et al. The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. J. Cell Biol. 108: 1799-1806, 1989. PubMed: 2715180
26075: Patrick J, et al. BC3H1細胞Acetylcholine receptor metabolism in a nonfusing muscle cell line. J. Biol. Chem. 252: 2143-2153, 1977. PubMed: 845167
