AMJ2-C11細(xì)胞

年限： 10 weeks old

ATCC Number： CRL-2456?

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 單核細(xì)胞/巨噬細(xì)胞

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

數(shù)量： 大量

品系： C57BL/6J

生長狀態(tài)： 懸浮生長，少量貼壁

器官來源： 肺

AMJ2-C11細(xì)胞Designations: AMJ2-C11

Depositors: AV Palleroni

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells

Organism: Mus musculus deposited as mouse

Morphology: macrophage

Source: Organ: lung

Strain: C57BL/6J

Cell Type: macrophage (alveolar); infected with J2 virus

Cellular Products: interleukin-6 (interleukin 6, IL-6)

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Antigen Expression: AMJ2-C11細(xì)胞MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 -

Age: 10 weeks old

Gender: female

Comments: AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. [48933]

Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.

Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.

AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP).

AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.

The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.

Constitutive expression of MHC-class-II antigens was low on AMJ2-C11 but was increased following exposure to rMuIFN-gamma.

The cell line did not secrete substantial amounts of IL-1 or TNF but did secrete large amounts of IL-6.

The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS. [48934]

Propagation: AMJ2-C11細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Firmly tap the flask against palm of hand to dislodge any attached cells and transfer along with the floating cells into new flasks.

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 48933: Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973

48934: Palleroni AV, et al. AMJ2-C11細(xì)胞Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450