L-132細胞
運輸方式： 凍存運輸
器官來源： 其他
ATCC Number： CCL-5?
數量： 大量
生長狀態： 貼壁生長
細胞形態： 上皮樣
是否是腫瘤細胞： 0
物種來源： 人
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Designations: L-132
Depositors: EV Davis
L-132細胞Biosafety Level: 2 [Cells contain Human papillomavirus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: HeLa contaminant
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
L-132細胞THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes: G6PD, A
HeLa Markers: Y
Comments: This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio:L-132細胞 A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes. Remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708
22127: Erfle V, Mellert W. Human cell line LC5 and its use. US Patent 5,582,967 dated Dec 10 1996
22222: . . Fed. Proc. 19: 386, 1960.
26042: . . Science 133: 2059, 1961.
32706: L-132細胞Tresnan DB, et al. Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J. Virol. 70: 8669-8674, 1996. PubMed: 8970993
