M. dunni細胞

ATCC Number： CRL-2017?

生長狀態： 貼壁生長

年限： *****

運輸方式： 凍存運輸

細胞類型： 成纖維細胞

是否是腫瘤細胞： 0

物種來源： 其他

細胞形態： 成纖維樣

數量： 大量

器官來源： 皮膚

M. dunni細胞Designations: M. dunni (Clone III8C)

Depositors: M Lander, SK Chattopadhyay

Originators: TC Hsu

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus dunni

Morphology: fibroblast

Source: Organ: skin

Cell Type: fibroblast

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Age: M. dunni細胞*****

Gender: female

Comments: Clone IIIC8 was derived from tail skin of a normal female Mus dunni mouse.

This line supports the replication of all four classes of MuLV, i.e., ecotropic, xenotropic, amphotropic and mink cell focus forming (MCF).

The cells exhibit cytopathic effects when infected with most ecotropic and MCF viruses.

They are resistant to infection by Moloney MuLV (presumably due to post translational modifications of the receptor), and lack most endogenous MuLV sequences.

To maintain the cells as confluent cultures (in viral assays, for example), use RPMI 1640 or Dulbecco's modified Eagle's medium.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: M. dunni細胞37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Twice per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove.

Add another 1 to 2 ml of fresh trypsin solution, and allow the cells to sit at 37C (or at room temperature) until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 22562: M. dunni細胞Lander MR, Chattopadhyay SK. A Mus dunni cell line that lacks sequences closely related to endogenous murine leukemia viruses and can be infected by ectropic, amphotropic, xenotropic, and mink cell focus-forming viruses. J. Virol. 52: 695-698, 1984. PubMed: 6092693