N1E-115細胞
器官來源： 大腦
數量： 大量
生長狀態： 貼壁生長
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
細胞形態： 神經母細胞
品系： A/J
ATCC Number： CRL-2263?
相關**： 神經母細胞瘤
Designations: N1E-115
Depositors: E Richelson
N1E-115細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: loosely adherent
Organism: Mus musculus
Morphology: neuroblast
Source: Organ: brain
Strain: A/J
Disease: neuroblastoma
Cell Type: neuroblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Receptors: acetylcholine, muscarinic m1; acetylcholine, muscarinic m2; vasoactive intestinal peptide (VIP) [22467]
adenosine; angiotensin II; bradykinin; enkephalin; glucagon; histamine H1; 5-hydroxytryptamine (serotonin, 5HT3); neurotensin; prostaglandin E; somatostatin; N1E-115細胞thrombin [22467]
Cytogenetic Analysis: modal number = 192
Comments: The N1E-115 cell line was established in 1971 by T. Amano, E. Richelson, and M. Nirenberg by cloning the C-1300 spontaneous mouse neuroblastoma tumor, C-1300. [22584]
The clone N1E was subcloned by isolation of single cells on glass shards. [23229]
The adrenergic clone N1E-115 exhibits high levels of activity of the enzymes tyrosine hydroxylase and acetylcholinesterase which are necessary for neurotransmitter synthesis but are almost devoid of choline acetyltransferase. [22584]
These cells contain 14 receptors for neurotransmitters. [22467]
The cells can be used to study neurotransmitters and their receptors with radioligand binding, second messenger synthesis and electrophysiological changes.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose (without sodium pyruvate), 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; N1E-115細胞carbon dioxide (CO2), 5%;if DMEM formulation contain 1.5 g/L sodium bicarbonate.
Temperature: 37.0°C
Subculturing: Protocol: 1. Remove medium, rinse with Modified Puck's Saline D1 solution: Glucose 5.5 mM,KCl 5.4 mM,Sucrose 58.4 mM,Na2HPO4-7H2O 0.17 mM,NaCl 138 mM,KH2PO4 0.22 mM.Adjusted to a pH of 7.4?0.05 with 0.1 N NaOH or 0.N HCl Adjusted to an osmomolality of 340?5 mosM with either sucrose or H20 to decrease or increase the osmomolality. 2. Allow flasks to remain at room temperature until cells detach. 3. To remove saline, add fresh culture medium, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. 4. iscard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 5. Place culture vessels in incubators at 37?C
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 36 hrs
Related Products: recommended serum:ATCC 30-2020
References: 21897: . N1E-115細胞Methods in neurotransmitter receptor analysis. New York: Raven Press; 1990.
22467: Richelson E. The use of cultured cells in the study of mood-normalizing drugs. Pharmacol. Toxicol. 66 suppl.3: 69-75, 1990. PubMed: 2179933
22584: Amano T, et al. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase- acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). Proc. Natl. Acad. Sci. USA 69: 258-263, 1972. PubMed: 4400294
23229: Richelson E. Regulation of tyrosine hydroxylase activity in mouse neuroblastoma clone N1E-115. J. Neurochem. 21: 1139-1145, 1973. PubMed: 4148612
