Hs27細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
器官來源： 皮膚
年限： newborn
細(xì)胞形態(tài)： 成纖維樣
生長狀態(tài)： 貼壁生長
細(xì)胞類型： 成纖維細(xì)胞
ATCC Number： CRL-1634?
相關(guān)**： 正常
是否是腫瘤細(xì)胞： 0
物種來源： 人
Designations: Hs27
Depositors: RB Owens
Hs27細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin; foreskin
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Hs27細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12,14
D16S539: 11,13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 6,8
vWA: 18
Hs27細(xì)胞Isoenzymes: G6PD, A
Age: newborn
Gender: male
Ethnicity: Black
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Hs27細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 32555: Suss-Toby E, et al. Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc. Natl. Acad. Sci. USA 93: 8413-8418, 1996. PubMed: 8710885
