4B2細胞

運輸方式： 凍存運輸

數量： 大量

ATCC Number： HB-196?

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態： 懸浮生長

細胞形態： **樣

**類型： IgG2a

Designations: 4B2

Depositors: RM Steinman

Isotype: IgG2a

Biosafety Level: 1

4B2細胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 4B2細胞immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with human T cells.

Spleen cells were fused with P3U1 myeloma cells.

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 4B2細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 21881: . Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ.4B2細胞 Press; 1987.