356-1細胞
細胞形態： **樣
**類型： IgG2a; kappa light chain
是否是腫瘤細胞： 0
物種來源： 小鼠
數量： 大量
ATCC Number： HB-181?
生長狀態： 懸浮生長
運輸方式： 凍存運輸
356-1細胞Designations: 356-1
Depositors: BS Prabhakar
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 356-1細胞immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); also reacts with heart muscle (myocardium); neutralizing
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Tumorigenic: Yes
Comments: Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 144).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a moderately conserved epitope on the JVB strain of CB4.
The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit.
Neutralizes many human isolates of CB4.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 356-1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: immunogen:ATCC VR-184
References: 1328: Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593
1329: Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617
23230: Ungar BL, et al. Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons. J. Infect. Dis. 153: 570-578, 1986. PubMed: 3950440
58148: Webb SR, et al. 356-1細胞Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930
