SF1-1.1.10細胞

運輸方式： 凍存運輸

數量： 大量

ATCC Number： HB-159?

生長狀態： 懸浮生長

細胞形態： **樣

**類型： IgG2a

是否是腫瘤細胞： 0

物種來源： 小鼠

Designations: SF1-1.1.10

SF1-1.1.10細胞Depositors: AM Stall

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: SF1-1.1.10細胞immunoglobulin; monoclonal antibody; against H-2 K d

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Comments: Animals were immunized with spleen cells from BALB/c mice.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody does not react with the b, f, k, p, q, s or v H-2 haplotypes.

SF1-1.1.10細胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. SF1-1.1.10細胞Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.