SC-71細(xì)胞
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
ATCC Number： HB-277?
細(xì)胞形態(tài)： **樣
**類型： IgG1
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
Designations: SC-71
Depositors: S Schiaffino
Isotype: IgG1
SC-71細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rat type 2A myosin heavy chain
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SC-71細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with bovine fetal skeletal muscle myosin.
Spleen cells were fused with NSO myeloma cells.
The antibody reacts with type 2A myosin heavy chain in Western blots, and type IIA rat muscle fibres by immunofluorescence.
The cells were deposited on behalf of Dr. Schiaffino by Regeneron Pharmaceuticals, Inc.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: SC-71細(xì)胞Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 23384: Bottinelli R, et al. Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle. J. Physiol. 437: 655-672, 1991. SC-71細(xì)胞PubMed: 1890654
