E13 161-7細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 大鼠
ATCC Number： HB-215?
細胞形態： **樣
**類型： IgG2a , IgG2a
生長狀態： 懸浮生長
數量： 大量
Designations: E13 161-7
E13 161-7細胞Depositors: IL Weissman
Isotype: IgG2a , IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse stem cell antigen 1 (Sca-1)
Permits/Forms: E13 161-7細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with a pre-T cell hybridoma.
Spleen cells were fused with P3X63 myeloma cells.
The antibody reacts with an antigen expressed on mouse hematopoietic stem cells.
Among bone bone marrow cells that are negative for lineage specific markers and have low expression of the Thy-1 antigen, the Sca-1 antigen identifies a virtually pure population of multilineage hematopoietic stem cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
0.05 mM 2-mercaptoethanol
E13 161-7細胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1787: Aihara Y, et al. E13 161-7細胞An attempt to produce ``pre-T'' cell hybridomas and to identify their antigens. Eur. J. Immunol. 16: 1391-1399, 1986. PubMed: 3096743
1789: Spangrude GJ, et al. Purification and characterization of mouse hematopoietic stem cells. Science 241: 58-62, 1988. PubMed: 2898810
