E.H. IV (Elaine IV)細胞
運輸方式： 凍存運輸
細胞類型： B**細胞
是否是腫瘤細胞： 0
物種來源： 人
生長狀態(tài)： 懸浮生長
ATCC Number： CCL-104?
相關**： 其他**
細胞形態(tài)： **樣
數量： 大量
E.H. IV (Elaine IV)細胞器官來源： 外周血
年限： 20 years
Designations: E.H. IV (Elaine IV)
Depositors: W Henle
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: infectious mononucleosis
Cell Type: E.H. IV (Elaine IV)細胞B lymphoblast;
Cellular Products: Epstein-Barr virus (EBV)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: partially resistant to poliovirus and vesicular stomatitis viruses
Isoenzymes: G6PD, B
Age: 20 years
Gender: female
Ethnicity: Caucasian
Comments: The cells carry and produce Epstein-Barr virus (EBV).
EBNA positive.
Biological Safety CLASS II.
Propagation:E.H. IV (Elaine IV)細胞 ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cultures can be maintained by addition of fresh medium or by replacement of medium by sedimentation. Establish new cultures by seeding in fresh medium at 5 X 10 exp5 viable cells/ml.
References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
26274: Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. E.H. IV (Elaine IV)細胞JAMA 203: 205-209, 1968. PubMed: 4864269
