RBA細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來源： 乳腺

ATCC Number： CRL-1747?

相關(guān)**： 腺癌

數(shù)量： 大量

品系： Sprague-Dawley

細(xì)胞類型： 其他細(xì)胞類型

年限： 50 days *****

是否是腫瘤細(xì)胞： 0

RBA細(xì)胞物種來源： 褐鼠

Designations: RBA

Depositors: LA Cohen

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus

Morphology: epithelial

Source: Organ: mammary gland

Strain: Sprague-Dawley

Disease: adenocarcinoma

Cell Type: chemically induced

Cellular Products: keratin

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Receptors: androgen receptor

glucocorticoid

insulin

androgen receptor, positive; glucocorticoid; insulin

Tumorigenic: Yes

Age: 50 days *****

Gender: female

Comments: The cells do not possess significant amounts of estradiol receptors.

The cells contain an activated H-ras (ras) oncogene.

The line lacks vimentin and reacts strongly with anti keratin antibodies (evidence of epithelial origin).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

RBA細(xì)胞Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Doubling Time: 18 to 20 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22313: Cohen LA, et al. Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture. In Vitro 10: 51-62, 1974. PubMed: 4220124

22669: Cohen LA, Karmali RA. Endogenous prostaglandin production by established cultures of neoplastic rat mammary epithelial cells. In Vitro 20: 119-126, 1984. PubMed: 6423517

22689: Vignon F, et al. RBA細(xì)胞Hormonal regulation in two rat mammary cancer cell lines: glucocorticoid and androgen receptors. Mol. Cell. Endocrinol. 13: 191-202, 1979. PubMed: 109329