E-8細胞

生長狀態： 懸浮生長

細胞形態： **樣

**類型： IgG1; kappa light chain

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-10179?

數量： 大量

E-8細胞Designations: E-8

Depositors: PH Klesius

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Cellular E-8細胞Products: immunoglobulin; monoclonal antibody; against heavy chain of channel catfish immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Spleen cells were fused with mouse myeloma cells.

The antibody is specific for the heavy chain of channel catfish immunoglobulin.

A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (see ATCC 56-X) is necessary for successful initiation of a culture from a thawed ampule.

Propagation: E-8細胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 45%; fetal bovine serum, 45%; DMSO, 10%

Related Products: Recommended medium E-8細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

feeder layer cells:ATCC 56-X